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Microbial Community Composition and Diversity via 16S rRNA Gene Amplicons: Evaluating the Illumina Platform

Figure 4

Correspondence of phylogenetic distance between 454 and Illumina.

This dendrogram included 24 replicates of our test sediment sample sequenced on the Illumina platform as well as the same sample sequenced on a 454 machine. In addition, 9 soda lakes samples that were equally sequenced on both technologies are included. For the soda lakes, the letters correspond to different systems whereas the numbers correspond to different sampling dates. Using the UniFrac metric to compute a distance matrix, a hierarchical clustering is performed following the UPGMA method. The detail of the taxonomic composition can be seen in S7 Fig.

Figure 4