Microbial Community Composition and Diversity via 16S rRNA Gene Amplicons: Evaluating the Illumina Platform
Every one of our sediment sample replicates, i.e. the triplicate two-step PCR, the one-step PCR and the updated chemistry are separated into three size fractions (low, medium, high) according to the peaks identified in Fig. 2. Fragments below 446 bp are exclusively originating from archaea. The second peak between 447 and 464 bp contains, for instance, contains a majority of the Chloroflexi. The last group above 465 bp holds most of the Bacteroidetes. Other species such as Proteobacteria or Firmicutes are found spanning a size range in the environmental sample.