Role of Tissue Factor in Mycobacterium tuberculosis-Induced Inflammation and Disease Pathogenesis
Macrophages (∼0.5×105 cells) were either stimulated with live M.tb (10 CFU/cell) or 10 µg/ml of gamma-irradiated M.tb (γ-M.tb) for overnight in antibiotic free RPMI complete medium. Macrophages were also stimulated with LPS (1 µg/ml) as a positive control for 6 h. At the end of the treatment, TF activity was measured in FX activation assay. * denotes significantly different from unstimulated controls. (* p<0.05; ** p≤0.001; *** p<0.0001) as obtained by Students t-test. Data are mean ± SEM (n = 6–17).