Silica Nanoparticles as the Adjuvant for the Immunisation of Mice Using Hepatitis B Core Virus-Like Particles
Figure 6
Proliferative response and cytokine production after immunisation of BALB/c mice with HBc VLPs formulated with different adjuvants in the presence or absence of MPL and splenocyte stimulation in vitro.
A – Stimulation indexes (SI), B – interferon (IFN)-γ level in culture media, C – IL-2 level in culture media. Splenocytes were isolated at day 10 after subcutaneous immunisation and stimulated with HBc VLPs. SI values are presented as the mean stimulation indexes for triplicate wells ± standard deviation (SD). The cytokine production values are gained from duplicate wells ± SD. MPL (alone) – control group, mice immunised with 10 µg of MPL in PBS. Naive – control group or unstimulated in vivo mice.