Melissa officinalis Extract Inhibits Laser-Induced Choroidal Neovascularization in a Rat Model
Expression of mRNAs for vascular endothelial growth factor (VEGF), matrix metalloproteinase (MMP)-2, and MMP-9 determined by real-time quantitative polymerase chain reaction (PCR) were evaluated in human retinal pigment epithelial (ARPE-19) cells (A, C, E) and in human umbilical vein endothelial cells (HUVECs) (B, D, F). (A, B) The relative VEGF mRNA levels, which are induced by tertiary-butylhydroperoxide (t-BH), were reduced significantly with cotreatment of 25 µg/mL of M. officinalis extract. (C, D) The expression of MMP-2 mRNA did not change in the t-BH treated cells, nor did that in the t-BH and M. officinalis extract treated cells. (E, F) The relative MMP-9 mRNA levels decreased significantly with cotreatment of 25 µg/mL of M. officinalis extract. Data are presented as mean ± standard deviation. #, *indicate significant difference (P<0.05) in comparison to negative control or the t-BH alone group, respectively.