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Cleavage of Histone 3 by Cathepsin D in the Involuting Mammary Gland

Figure 4

The observed H3 cleavage site (depicted by green color in A) by involution-derived mCatD.

Theoretically predicted cleavage sites are also indicated (red in A). The in vitro cleavage of the recombinant H3.3 protein by ID2-derived mCatD and CatL is also indicated in Fig. 4B, with the total cell lysate of the mouse mammary gland at involution day 2 included as reference. The NH2 terminal sequence of H3 cleavage product (marked by *) is depicted in C.

Figure 4

doi: https://doi.org/10.1371/journal.pone.0103230.g004