High Metastaticgastric and Breast Cancer Cells Consume Oleic Acid in an AMPK Dependent Manner
Figure 6
AMPK is involved in the OA-mediated protein expressions of fatty acid metabolism enzymes.
The gastric carcinoma cells and breast cancer cells were cultured with 0.5% BSA or 400 µM of BSA-bound oleic acid with 5 µM of Compound C or with 100 µM of AICAR for 48 h. The levels of pAMPKα (Thr172), the total AMPKα, pACC (Ser79), the total ACC, CPT1α, MCAD and ATGL were determined by western blot analysis using 50 µg of the total proteins from each sample. A representative blot is shown. (B) The cells transfected with control-siRNA or AMPKα1-siRNA (24 hours) were treated with 0.5% BSA or 400 µM of BSA-bound oleic acid for another 48 hours. The expressions of pAMPKα (Thr172), the total AMPKα, pACC (Ser79), the total ACC, CPT1α, MCAD and ATGL were determined by western blot analysis.