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Evaluation of a Novel Magneto-Optical Method for the Detection of Malaria Parasites

Figure 3

Hemozoin crystals observed by electron microscopy in-situ and after isolation.

The transmission electron microscopy (TEM) image in panel A and scanning electron microscopy (SEM) images in panels B, C and D show hemozoin crystals extracted from the samples previously used for the MO measurements. The granular background of the SEM images comes from the gold coating of the glass substrate. Individual crystals in panels C and D are marked with asterisks. The length of the black bar at the bottom right corner of each panel is 500 nm. The typical length of the extracted hemozoin crystals ranges from 200 nm to 500 nm. For comparison, a TEM image of synthetic hemozoin crystals used in our previous MO study [15] is shown in panel E. These synthetic crystals have more elongated shape with a typical length of 500–900 nm. Panels F and G display TEM images of intact infected erythrocytes in the schizont stage. Distinct components of the parasite and the erythrocyte can be observed including the erythrocyte membrane (EM), parasite membrane (PM), food vacuole membrane (FVM), merozoites (M), hemoglobin transport vesicles (HbTV), knobs (K) and hemozoin (Hz).

Figure 3