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Proteomic Changes during B Cell Maturation: 2D-DIGE Approach

Figure 3

Unsupervised clustering of B cell samples on the basis of protein profiles.

IM-B Ramos samples clustered according to time after anti-IgM stimulation (1–120 h) and were distinctive from early pre-B (RS4;11, 380 and REH), pre-B (697, Nalm-1 and Nalm-6) and plasma cell U-266 samples. The degree of similarity in profiles is shown by colour (base-two logarithmical scale below the figure). Red and green indicate high and low levels of protein expression in relative to internal standard. The average protein abundance of the 175 identified proteins (labelled with Cy3 and Cy5 dyes) is calculated relative to internal standard (labelled with Cy2 dye). Cluster numbers (1–19) are shown to the right and refer to those in Table 1, Table 2, Table 3, Table S2 and Table S4.

Figure 3