Advertisement
Browse Subject Areas
?

Click through the PLOS taxonomy to find articles in your field.

For more information about PLOS Subject Areas, click here.

< Back to Article

Fatty Acids are Key in 4-Hydroxy-2-Nonenal-Mediated Activation of Uncoupling Proteins 1 and 2

Figure 2

Dependence of UCP-mediated proton conductance on HNE concentration.

Membranes from E. polar lipid were reconstituted with 15% arachidonic acid and 10.5 µg/(mg lipid) UCP1 (charge 19). Buffer solution contained 50 mM Na2SO4, 10 mM Tris, 10 mM MES, 0,6 mM EGTA, at pH 7.5 and T = 32°C. Data points represent mean ± standard deviation from at least 3 independent experiments. The data points were fitted following Michaelis-Menten kinetics as described in the text.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0077786.g002