System for Stable β-Estradiol-Inducible Gene Expression in the Moss Physcomitrella patens
Figure 6
Time course responsiveness to β-estradiol in the GX6-NGG and GX8-NGG lines.
(A, B) Relative transcript levels of NGG in protonemata of GX6-NGG#63 (A) and GX8-NGG#4 (B) lines. Relative transcript levels of NGG are normalized to TUA1 and then standardized to the normalized transcript level of protonemata at 0 h. Protonemata were immersed in water with (+: circles) 1 µM β-estradiol and collected after 0, 1, 3, 6, 12, 24, and 48 h. As a negative control, protonema cultivated without (-: crosses) β-estradiol were collected after 0, 6, 12, and 48 h. Error bars indicate SD of the mean (n = 3).