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Urinary Fetuin-A Is a Novel Marker for Diabetic Nephropathy in Type 2 Diabetes Identified by Lectin Microarray

Figure 2

SSA-Agarose column choromatography performed in the 4 patients with type 2 diabetes.

A. The concentrated urine samples were applied to SSA-Agarose column, washed with PBS and eluted with 0.2 M lactose. B. The effluents from the patients manifested with various albuminria and GFR stages, A3G3 and A3G4, were subjected to SDS-PAGE and stained with Coomassie Brilliant Blue. The bands were visualized and they were subjected to liquid chromatography-tandem mass spectrometer (LC/MS-MS) analysis.

Figure 2