Resveratrol Inhibits CD4+ T Cell Activation by Enhancing the Expression and Activity of Sirt1
Cells were then treated with Res (10µg/ml) for additional 8 hours. (a) c-Jun acetylation in the lysates of treated cells was determined by immunoprecipitation with anti-c-Jun antibody and western blotting with anti-acetyl-lysine Abs (top panel). The expression levels of c-Jun (middle panel) and Sirt1 (bottom panel) in the whole cell lysate were confirmed by western blotting. (b) The levels of acetylated c-Jun were quantified and normalized with total c-Jun and its relative levels are shown. Error bars represent data from three independent experiments (mean + SD).