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PhagoSight: An Open-Source MATLAB® Package for the Analysis of Fluorescent Neutrophil and Macrophage Migration in a Zebrafish Model

Figure 9

Mean neutrophil volume relative to its position in an area anterior to the wound region.

The field of view was split into 25 adjacent regions each containing 20 MATLAB® columns, with higher numbers denoting neutrophils closer to the wound region. The volume was averaged in each band and normalised to the mean of the data set for each larva, which were then pooled in the final analysis. Two sets of thresholds were used. Analysis A uses higher thresholds than analysis B.

Figure 9