Improved Bone Morphogenetic Protein-2 Retention in an Injectable Collagen Matrix Using Bifunctional Peptides
A bifunctional peptide (BC-1) was synthesized containing Peptide B-17 and a collagen-binding peptide with a short amino acid linker. rhBMP-2 was mixed with or without the bifunctional peptide and added to a collagen gel. The bifunctional peptide enhanced the retention of rhBMP-2 to the collagen gel (no peptide, EC50 = 5.5 nM; BC-1, EC50 = 0.41 nM). Data are presented as the absorbance read at 405 nm.