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Improved Bone Morphogenetic Protein-2 Retention in an Injectable Collagen Matrix Using Bifunctional Peptides

Figure 6

Peptide-mediated capture of BMP-2 from spiked TBST or plasma.

The biotinylated peptides were coated on streptavidin plates and incubated in TBST (A) or plasma (B) with nanomolar concentrations of rhBMP-2 for 1 h. BMP-2 binding was analyzed using antibody and p-NPP detection. The peptides captured more rhBMP-2 from solution than the no peptide control. Data are presented as the absorbance read at 405 nm.

Figure 6