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Improved Bone Morphogenetic Protein-2 Retention in an Injectable Collagen Matrix Using Bifunctional Peptides

Figure 5

Peptide cross-reactivity to other growth factors.

Peptides B-17 (A) and B-18 (B) were immobilized on a streptavidin-coated plate, and a range of concentrations of growth factors in the TGF-β superfamily were titrated onto the plate in TBST for 1 h. Growth factor binding was analyzed using antibody and p-NPP detection. Both peptide B-17 and B-18 bound to rhBMP-2, rhBMP-6, and rhBMP-7. Peptide B-17 also cross-reacted with rhBMP-3, rhBMP-5, and rhBMP-12. The peptides had lower affinities for all other growth factors tested. Data are presented as the absorbance read at 405 nm.

Figure 5