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Improved Bone Morphogenetic Protein-2 Retention in an Injectable Collagen Matrix Using Bifunctional Peptides

Figure 4

BMP-2 Binding to Peptide.

Biotinylated peptides were immobilized on streptavidin-coated plates and incubated with a range of rhBMP-2 concentrations in TBST for 1 h. BMP-2 binding was analyzed using antibody and p-NPP detection. Peptides B-6, B-17, and B-18 had the highest binding affinities for rhBMP-2. Peptide N-1 is a negative control that binds hexokinase. Data are presented as the absorbance read at 405 nm.

Figure 4