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Rapid Global Fitting of Large Fluorescence Lifetime Imaging Microscopy Datasets

Figure 5

Global and pixel-wise analysis of simulated TCSPC polarisation resolved image data.

Simulated polarisation resolved TCSPC data was generated with fluorescence lifetimes 3.0 and 1.2 ns and rotational correlation times of 30 ns and 1.0 ns. The simulated data was generated with the total initial anisotropy set to 0.4 across the image with the initial anisotropy contribution of the short component equal to 0.1, 0.2 and 0.3 in three bands from top to bottom across the image. (A) False colour images of the recovered initial anisotropy contribution for the long (left) and short (right) correlation time components analysed pixel-wise (top) and with global fitting (bottom); Histograms of estimates of the initial anisotropy contribution of the (B) long and (C) short correlation time components analysed pixel-wise (dashed lines) and with global fitting (solid lines).

Figure 5

doi: https://doi.org/10.1371/journal.pone.0070687.g005