Rapid Global Fitting of Large Fluorescence Lifetime Imaging Microscopy Datasets
Global analysis was performed across a multiwell plate dataset with HeLa cells expressing ECFP-Gag and EYFP-Gag with increasing levels of a NMT inhibitor using a bi-exponential donor FRET model. A) representative images from each inhibitor dose showing distribution of fraction Gag-CFP undergoing FRET. B) plot of fraction of Gag-CFP undergoing FRET against inhibitor concentration, averaged across wells with fitted dose-response curve. Error bars indicate 95% confidence intervals across wells. This dataset was collected as part of a previous study . White scale bar represents 100 µm.