Rapid Global Fitting of Large Fluorescence Lifetime Imaging Microscopy Datasets
Global analysis of a multiwell plate with varying concentrations of fluorescent dyes.
Global analysis was applied to a multiwell plate with varying concentrations of the fluorescent dyes Rhodamine B and Rhodamine 6G using a bi-exponential model. The relative concentration of Rhodamine 6G reduces across pairs of columns as described in the text. The dataset contains four fields FOV per well. A) plate map showing the measured fractional contribution of Rhodamine 6G for a representative FOV in each well. B) plot of the actual Rhodamine 6G contribution against measured contribution (crosses). C) plot of measured lifetime using a single exponential fit against actual Rhodamine 6G concentration. This dataset was collected as part of a previous study .