Rapid Global Fitting of Large Fluorescence Lifetime Imaging Microscopy Datasets
(A) Fractional core activity of the four cores while performing the fit in Experiment 1, colour coded by algorithm stage as shown in the flowchart in (B) which shows the main stages of the algorithm. (C) CPU time spent on the different algorithm stages in Experiment 1. (E) Memory requirements for a global fit against image number for (blue) a five frame time gated FLIM dataset, (red) a TCSPC dataset and (green) a two channel polarisation resolved dataset. Numbers exclude the memory required for the MATLAB runtime engine (300 MB).