Aureolib — A Proteome Signature Library: Towards an Understanding of Staphylococcus aureus Pathophysiology
(A) S. aureus COL was cultivated in synthetic medium at 37°C and treated with 10 mM H2O2 at OD500 0.5 (0 min). (B) Protein synthesis profiles were normalized using total normalization and standardized (z-score). Significantly changed synthesis profiles were determined by ANOVA (α = 0.1, distribution based on all permutations, absolute threshold of 2.5). Based on their expression kinetics in response to H2O2, proteins can be allocated to three groups: proteins which are induced during the acute phase immediately after imposition of stress (red lines), proteins induced during resumption of growth (blue lines) and proteins highly expressed in the tolerance phase (green lines).