Discovery and Characterization of a Novel Carlavirus Infecting Potatoes in China
Figure 3
Serological relationships among different potato carlaviruses according to the western blotting analysis.
The rabbit polyclonal antiserum raised against the coat protein (CP) of Potato virus H–Ho and the detection antibodies (Agdia, Indiana USA) against Potato virus M (PVM), Potato virus S (PVS), and Potato latent virus (PoLV) were used in the analysis. The purified PVH (PurPVH), purified pET-CPPVH expression product (CPPVH-his), pGEX-CPPVM expression product (CPPVM), pGEX-CPPVS expression product (CPPVS), and the lyophilized positive control for PoLV (PoLV) (Agdia, Indiana USA) were used in the tests. Crude extracts from uninfected leaves of potato and Nicotiana glutinosa (N. g.) were used as the negative controls.