Identification and Mapping of Linear Antibody Epitopes in Human Serum Albumin Using High-Density Peptide Arrays
The figure shows two examples example of epitope sequences identified using ANOVA followed by Tukey’s HSD post-hoc analysis. The leftmost column shows overlapping 15-mer peptides from HSA from position 510–527 in figure 3A and from position 309 to 333 in figure 3b. The rightmost column highlights the amino acids identified as being important for antibody binding (dashes indicate non-important positions). The important amino acids were determined on the p<0.01 level in the HSD post-hoc analysis.