Identification and Mapping of Linear Antibody Epitopes in Human Serum Albumin Using High-Density Peptide Arrays
Bar chart displaying the fluorescence signal obtained from binding of polyclonal anti-HSA to 15-mer peptides with 14 residue overlap (average signal from 5 copies of each peptide). A: peptides from HSA, B: peptides from BSA and C: peptides from RSA. The peptides are numbered on the x-axis according to the position of their n-terminal residue in the protein sequence. The y-axis denotes the average intensity of the signal (AU) after background subtraction.