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Light Scattering Properties Vary across Different Regions of the Adult Mouse Brain

Figure 3

Effects of wavelength on optical transmittance.

3A: Optical transmittance in the MNTB as a function of tissue thickness and optical wavelength. The three color-coded data sets represent corresponding measurements with light of three different optical wavelengths (blue (453 nm), green (528 nm), and red (940 nm)). Longer-wavelength light penetrates tissue deeper, resulting in a higher transmittance at any given tissue thickness. 3B: Effects of light wavelength on transmittance in two brain areas (MNTB and VNTB). The effective attenuation coefficient decreases with wavelength for the three wavelengths tested. MNTB measurements are represented by round symbols while VNTB measurements are represented by square symbols. Measurements in the three different colors are indicated by the color-code of the symbols.

Figure 3