CLUSTOM: A Novel Method for Clustering 16S rRNA Next Generation Sequences by Overlap Minimization
Figure 4
Taxonomy-based accuracy evaluation.
The clustering results of CLUSTOM, NW-DOTUR-AL, ESPRIT-Tree, NAST-mothur-AL and NW-mothur-AL were evaluated using the 16S–SILVA sequences. (A–B): The number of clustered OTUs was examined at the species (3% dissimilarity; A) and genus (5%; B) levels for every OTU size (number of sequences in a cluster). (C–D): The precision and recall statistics as well as their harmonic mean (F2) [25] were used to evaluate clustering results of the five programs at the two taxonomic levels.