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cDNA Cloning and Molecular Modeling of Procerain B, a Novel Cysteine Endopeptidase Isolated from Calotropis procera

Figure 1

Amplified fragment from a Calotropis procera cDNA pool using degenerate forward (based on first seven N-terminal amino acids ofprocerain B) and oligo dT(18) reverse primer.

Lane M represents Low range DNA ruler (Bangalore Genei). Lane 1 and 2 represents ∼ 0.75 kb cDNA fragment. One percent agarose gel stained with ethidium bromide.

Figure 1