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Altered Subcellular Localization of Heat Shock Protein 90 Is Associated with Impaired Expression of the Aryl Hydrocarbon Receptor Pathway in Dogs

Figure 5

Significant immunohistochemical staining of liver tissue.

Representative images of immunoreactivity in sections of formalin-fixed, paraffin-embedded liver samples from control dogs, dogs with extrahepatic and intrahepatic portosystemic shunts, and Irish Wolfhounds. Immunoreactivity against the aryl hydrocarbon receptor (AHR)(A), aryl hydrocarbon receptor nuclear translocator (ARNT)(B), hypoxia inducible factor 1alpha (HIF1A)(C), heat shock protein 90kDa alpha (cytosolic), class A member 1 (HSP90AA1)(D), and vascular endothelial growth factor A (VEGF)(E) is visible in variable intensity in the cytoplasm and/or nuclei of hepatocytes.

Figure 5

doi: https://doi.org/10.1371/journal.pone.0057973.g005