High Resolution Helium Ion Scanning Microscopy of the Rat Kidney
(A) Two adjacent endothelial cells from a glomerular capillary (GA-fixed, dehydrated using the extended methanol freeze-substitution protocol), imaged from the luminal side. The most striking features of these cells are the numerous, round fenestrations that are present over the entire cell surface. The raised ridges (arrows) represent the location of the tight junction between the two cells. Bar = 175 nm. (B) Higher magnification showing details of the fenestrations. In some of them, a substructure consisting of faint spokes like a bicycle wheel can be seen (arrows). Bar = 80 nm.