Rational and Evolutionary Engineering Approaches Uncover a Small Set of Genetic Changes Efficient for Rapid Xylose Fermentation in Saccharomyces cerevisiae
Figure 7
PHO13 deletion affects xylose fermentation differently in various strain backgrounds.
(a-d) Xylose fermentation parameters in three different xylose-assimilating strains and (e and f) growth patterns of the SR7 strain in various xylose concentrations: a, xylose consumption rate (g/h); b, specific xylose consumption rate (g/g cell/h); c, xylitol yield (g/g xylose); d, ethanol yield (g/g xylose); e, cell growth of SR7; f, cell growth of SR7 pho13Δ. All parameters were calculated at 36 h during 40 g/l xylose fermentation. An initial cell density was adjusted to 0.3 g/l (a-d) or 0.03 g/l (e-f). All experiments were duplicated.