Tobacco Mosaic Virus in the Lungs of Mice following Intra-Tracheal Inoculation
Figure 2
Detection of TMV antigen by immunohistochemistry in lungs of TMV-inoculated mice.
No immunodetection was observed in lung from a water-inoculated mouse (A), whereas cytoplasmically immunopositive cells located in inflammatory infiltrates present in interalveolar walls, most likely macrophages, were detected at day 1 (B) and day 3 (C) after intratracheal inoculation. Polyclonal rabbit anti-TMV antibody was used at a dilution of 1∶500, with hemaxylin counterstain. Magnification, 400X. Arrows show macrophages positive for TMV antigen detection.