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Unveiling Distribution Patterns of Freshwater Phytoplankton by a Next Generation Sequencing Based Approach

Figure 4

Boxplot showing ratios in taxonomic composition (at phylum level) as revealed by next generation sequencing (NGS) vs. microscopy.

Plots show the ratio between relative reads numbers and biovolumes (as determined by microscopy) for each phylum. (AM) Alinen Mustajarvi, (ER) Lake Erken, and (RI) Rimov Reservoir. A ratio above zero indicates that a specific phylum is preferentially detected by NGS whereas a ratio below zero indicates an over representation in the biovolume data relative to NGS. The part of the plot indicated in grey represents the area where the ratio is the result of that a phylum was only detected by either method.

Figure 4