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Engineered Drug Resistant γδ T Cells Kill Glioblastoma Cell Lines during a Chemotherapy Challenge: A Strategy for Combining Chemo- and Immunotherapy

Figure 3

Transduction of γδ T cells with lentivirus vector was performed on day 6, 7 and 8 of expansion culture (see text) with increasing MOI.

(a) On day +14 cells were incubated in media supplemented with 400 µM TMZ and viable cell counts were obtained for each MOI. Two separate experiments are shown. (b) Quantitative PCR analysis to measure P140KMGMT copy numbers of the bioengineered γδ T cells in the presence of increasing concentrations of TMZ, which are indicated in the figure.

Figure 3