Differential Effects of Bisphenol A and Diethylstilbestrol on Human, Rat and Mouse Fetal Leydig Cell Function
Testes were removed from human fetuses at 6.5 to 10.5 weeks of gestation or from mouse and rat fetuses at 12.5 and 14.5 dpc, respectively. For each fetus, both testes were cultured in control medium for 24 h (D0) and then one in the presence of 10−8 M BPA and the other one without for another 24 h. At the end of the culture, RT-PCR amplification was performed. Values are the mean ± SEM of INSL3 mRNA level normalized to β-Actin one, and expressed as percentage of control values from 7 (human), 5 (mouse) and 6 (rat) independent experiments. * p<0.05 in the statistical comparison between BPA-treated and control testes using the paired Wilcoxon’s non-parametric paired test.