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Estimating Microtubule Distributions from 2D Immunofluorescence Microscopy Images Reveals Differences among Human Cultured Cell Lines

Figure 3

Generation of 3D cell geometry (cell shape and nuclear shape) from real 2D slices of the microtubule and nucleus channels.

(A) Example of a real 2D cell image (tubulin channel) and its approximate bottom shape. (B) Cartoon of an X-Z projection of a cell on a substrate. (C) Example of a generated 3D cell shape containing 8 stacks (1.6 microns). (D) Illustration of inputs and outputs for the procedure.

Figure 3