Five Different Piscidins from Nile Tilapia, Oreochromis niloticus: Analysis of Their Expressions and Biological Functions
Fish cells (tilapia ovary (TO2) cell line) were treated with different doses of piscidin peptides (TP1∼5) for 24 h, followed by an MTS assay. Each concentration was repeated in eight wells for three independent experiments. Piscidin treatment affected cell viability in a dose-dependent manner. There were significant differences between treatment groups for (a) TP3 (40 µg/ml) and (b) TP4 (12 µg/ml) compared to 0 µg/ml treatment. There were significant differences between treatment groups for the lower concentrations in (c) TP2 (30 µg/ml) and TP5 (10 µg/ml) compared to 0 µg/ml treatment. But survival of cells treated with 100 µg/ml of TP1, -2, and -5 were still >80%. Data are from three separate experiments. Data (mean ± SE) with different letters significantly differ (p<0.05) among the experimental groups.