A Potential Peptide Therapeutic Derived from the Juxtamembrane Domain of the Epidermal Growth Factor Receptor
(A–B) The indicated cell line was plated overnight, then serum starved overnight and treated with TE-64562 for 24 hours. For the HMEC and MDA-MB-231 cells were treated in HMEC media. Representative dose response curves are shown from one experiment run in triplicate with error bars representing the standard error of the mean. In the legend, the mean EC50 values (± standard deviation from two to three independent experiments) derived from the nonlinear fit of dose response curves are shown (generated and fitted in Prism 5.0 GraphPad Software, Inc., USA). Also see Figure S2. (C) MDA-MB-231 (breast), A-549 (non-small cell lung), DLD-1 (colo-rectal) MIA-PaCa-2 (pancreatic) and SK-N-MC (neuroepithelioma, EGFR-null) cells were grown in soft agar containing 5% serum alone or treated with Tat peptide (20 µM) or Tat-645-62 peptide (10 or 20 µM) and allowed to grow for 2 weeks with addition of medium or medium containing peptide every 2 days. Means of the counts of four or more plates from at least two independent experiments are plotted. The significantly difference between the mean counts (*P<0.04) for TE-64562 treatment was assessed by comparison to untreated control.