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Immunization with a Hemagglutinin-Derived Synthetic Peptide Formulated with a CpG-DNA-Liposome Complex Induced Protection against Lethal Influenza Virus Infection in Mice

Figure 5

IgG production by a complex of Lipoplex(O) and conserved sequences corresponding to hH5N1 HA233 epitope.

Fourteen amino acid long conserved sequences were synthesized; the sequences correspond to hH5N1 HA233 epitope of the A/Vietnam/1203/2004 strain; they were obtained from influenza A virus H5N1 strains and influenza A virus subtypes. Three BALB/c mice were immunized i.p. three times with a complex of each peptide and Lipoplex(O). The antisera were collected 10 days after the final immunization, and then amounts of each peptide-specific total IgG (A,D), IgG1 (B) and IgG2a (C) were measured by ELISA. The ELISA plates were coated with hH5N1-HA 233 peptide.

Figure 5