Visualizing Escherichia coli Sub-Cellular Structure Using Sparse Deconvolution Spatial Light Interference Tomography
Figure 3
Three dimensional point spread function.
A) Comparison of raw and deconvolved. PSF in the x-y plane; the deconvolution process reduces the FWHM from 397 nm to 153 nm. B) Comparison of raw and deconvolved PSF in the x–z plane; the deconvolution process reduces the FWHM from 1218 nm to 357 nm. The dashed lines show the data and the circular markers indicate the Gaussian fit used to determine the FWHM.