Immunization with SARS Coronavirus Vaccines Leads to Pulmonary Immunopathology on Challenge with the SARS Virus
Serum neutralizing (neut) antibody and lung virus titers for each vaccine dosage group. A. Geometric mean serum antibody titer and standard error of the mean (S.E.) on day 56 for each vaccine dosage group for each mouse strain (Balb/c or C57BL/6). Five mice per group given 0.1 ml of vaccine intramuscularly on days 0 and 28. B. Geometric mean virus titer (log10 TCID50/g) and standard error of the mean (S.E.) in lungs on day 58 (two days after SARS-CoV challenge for each vaccine dosage group for each mouse strain. Seven to eight mice per group. Vaccines: Double inactivated whole virus, (DIV), β propiolactone inactivated whole virus (BPV), with alum (+A). Analyses: A. GMT for highest DIV dosage without alum greater for Balb/c than C57BL/6 p = .008 but not for alum p>.05. GMT for the BPV vaccine and live virus were not different for the two strains p>.05. B. GMT for PBS control mice were not different p>.05. GMT for DIV without alum and BPV with alum greater for C57BL/6 than Balb/c p = .004.