Immunization with SARS Coronavirus Vaccines Leads to Pulmonary Immunopathology on Challenge with the SARS Virus
Serum neutralizing (neut) antibody and lung virus titers for each vaccine dosage group. A. Geometric mean serum antibody titer as log2 and standard error of the mean (S.E.) on day 56 for each vaccine dosage group. Seven to eight mice per group. Vaccines: double inactivated whole virus (DIV), recombinant S protein (SV), viral-like particle vaccine (VLP), with alum (+A). Five mice per group were given 0.1 ml of vaccine intramuscularly on days 0 and 28. B. Geometric mean virus titer (log10 TCID50/g) and standard error of the mean (S.E.) in lungs on day 58 (two days after SARS-CoV challenge) for each vaccine dosage group. Analyses: A. GMT with compared to without alum: DIV p>.05, VLP p>.05, SV p = .001. GMT for different vaccine dosage: DIV with alum p = .007, DIV without alum p>.05, SV with alum p = .028, SV without alum p = .01. Multiple regression: GMT increased for alum p = .012 and dosage p<.001, for SV alum only p = .001. B. GMT for all DIV groups not different p>.05, GMT for SV group without alum p .008 and with alum p .023. GMT for VLP group is not different p>.05.