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Dimethyl Sulfoxide Promotes the Multiple Functions of the Tumor Suppressor HLJ1 through Activator Protein-1 Activation in NSCLC Cells

Figure 2

DMSO induces HLJ1 expression in concentration-dependent and time-dependent manners.

(A) Real-time quantitative RT-PCR reveals that HLJ1 mRNA expression was induced by DMSO in a concentration-dependent manner (0.1–2%, v/v) after 48 h incubation. (B) Concentration-dependent DMSO-induced HLJ1 expression at the protein level was confirmed by Western blot analysis. α-tubulin was a control for protein loading and transfer. (C) Time-dependent DMSO-induced HLJ1 expression at the mRNA level was verified by real-time quantitative RT-PCR. The results indicated that the mRNA level of HLJ1 was significantly increased after 2% DMSO treatment for 2 h. *P < 0.05, significantly different from the vehicle-treated control. (D) The protein expression level of HLJ1 after 2% DMSO treatment at the indicated time was detected by Western blot analysis. The result indicated that HLJ1 protein expression was time-dependent and was sustained with DMSO treatment for 48 h. α-tubulin was a control for protein loading and transfer.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0033772.g002