Caenorhabditis elegans Battling Starvation Stress: Low Levels of Ethanol Prolong Lifespan in L1 Larvae
L1 larvae were isolated as described in Figure 1. For each time point (T = 0, 3 and 7 days) two 100-ml cultures containing 1000 starved L1 larvae/ml were prepared in triplicate. The zero day time point consisted of starved L1 larvae one day after hatching; the 3 day and 7 day time points consisted of larvae incubated with 1 mM fully-deuterated ethanol. Protein was isolated and hydrolyzed and amino acids were derivatized and analyzed using GC-MS for deuterium incorporation as described in the “Methods” section. The M-15 and M-117 ions for glutamate and proline were analyzed and data are shown as the average of 3 separate trials analyzed twice by GC-MS. Asterisks indicate a p-value from Student's T-test of less than 0.05 when the 3-day and 7-day peaks were compared to the 0-day controls.