Caenorhabditis elegans Battling Starvation Stress: Low Levels of Ethanol Prolong Lifespan in L1 Larvae
L1 larvae (70,000) were isolated as described in Figure 1 and were incubated at 20°C in either M9 medium only or M9 medium supplemented with 4 mM, 17 mM, or 68 mM ethanol (panels A and B; legend for both in panel B) and 0.001, 0.01, 0.1, or 1 mM ethanol (panel C). Survival was scored every 48 hour until all larvae were dead. Mantel-Cox logrank analyses gave a p-value of <0.0001 for the difference between the control (no ethanol) and all concentrations of ethanol at 1 mM or above. The survival at 68 mM ethanol is significantly different from that at 17 mM ethanol in panel A (p = 0.0001) and in panel B (p = 0.001).