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An Interaction Network Predicted from Public Data as a Discovery Tool: Application to the Hsp90 Molecular Chaperone Machine

Figure 1

Workflow for the construction of the interactome of the human Hsp90 molecular chaperone machine (Hsp90Int).

(A) PPIs from online databases were compiled and edited in order to obtain consistent and uniform data. These data were enriched with manually curated information from the literature. (B) Physical interactomes were constructed and visualized with Cytoscape for the indicated species. (C) Components of the Hsp90 molecular chaperone machine were used as query proteins (colored nodes) to retrieve the corresponding PPI network for each organism. (D) HomoloGene IDs were assigned to the interactors in each network to identify human orthologs and new interolog interactions. (E) The complete sets of interologs were merged with the human network into the complete human Hsp90Int. The red and blue colors represent human and interolog interactions, respectively.

Figure 1