SMURF1 Amplification Promotes Invasiveness in Pancreatic Cancer
(A) Transfection of SMURF1 cDNA, or a catalytic mutant of SMURF1 (C699A) (SMURF1m), leads to overexpression (by Western blot) compared to empty vector control. SMURF1 overexpression levels, normalized to GAPDH, are indicated. (B) SMURF1 (but not SMURF1m) overexpression in NIH-3T3 cells leads to loss of contact inhibition (i.e. increased foci formation). Assays done in triplicate (mean +/− 1SD shown); *, P<0.05 (Student's t-test).