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Differential Gene Expression in the Siphonophore Nanomia bijuga (Cnidaria) Assessed with Multiple Next-Generation Sequencing Workflows

Figure 7

In situ characterisation of gene expression.

Expression analysis of isogroup03256 in developing nectophores (A) and gastrozooids (B). The transcript is localized in the basigaster, a region associated with nematogenesis, at the base of gastrozooids and detectable in very early stages of gastrozooid development (arrow). (C) Sense control with an unstained basigaster region. In situ hybridizations for nectophore specific transcripts have been performed successfully (data not shown). S: stem of the colony, b: basigaster region of the gastrozooid, pn: pneumatophore.

Figure 7