Differential Gene Expression in the Siphonophore Nanomia bijuga (Cnidaria) Assessed with Multiple Next-Generation Sequencing Workflows
Figure 4
Accumulation curves indicating the number of genes with significant differential expression (DE) when short reads are subsampled.
Number of DE sequences are plotted against subsampled library sizes considering the full reference (A) and the subset of sequences with the 3′-most NlaIII site (B). This enables comparison of significance across equivalent library sizes.